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子宫内膜非典型增生中TRF1、TRF2和H3K27me3的表达及意义

来源:用户上传      作者:郭慧 谭丽珊 梁春燕 吴立琦 陈玉英

  [摘要] 目的 探讨端粒重复序列结合因子1(TRF1)、端粒重复序列结合因子2(TRF2)和组蛋白H3K27三甲基化(H3K27me3)免疫组化在子宫内膜非典型增生组织中的诊断作用。方法 收集2016年3月至2018年11月广东省韶关市第一人民医院诊断的不伴子宫内膜非典型增生标本30例和子宫内膜非典型增生标本60例及子宫内膜样癌30例。利用免疫组化SP法检测TRF1、TRF2及H3K27me3在这些标本中的表达情况。结果 TRF1、H3K27me3、TRF2在子宫内膜非典型增生组中的表达率分别为35.00%(21/60)、43.33%(26/60)、71.67%(43/60),在不伴非典型子宫内膜增生组的表达率83.33%(25/30)、73.33%(22/30)、30.00%(9/30),在子宫内膜样癌组中的表达率为30.00%(9/30)、36.67%(11/30)、76.67%(23/30)。子宫内膜非典型增生与不伴非典型子宫内膜增生表达率比较,差异有统计学意义(P<0.05);子宫内膜非典型增生与子宫内膜样腺癌表达率比较,差异无统计学意义(P>0.05)。结论 子宫内膜非典型增生TRF1及H3K27me3低表达,TRF2高表达,联合TRF1、TRF2和H3K27me3有助于鉴别不典型子宫内膜增生与不伴有非典型子宫内膜增生,有助于子宫内膜非典型增生的早期诊断,推荐在常规病理工作中使用。
  [关键词] 子宫内膜非典型增生;TRF1;TRF2;H3K27me3;诊断
  [中图分类号] R364.3+1;R711 [文献标识码] A [文章号] 1673-9701(2022)13-0001-03
  [Abstract] Objective To investigate the effects of telomere repeat binding factor 1 (TRF1), telomere repeat binding factor 2 (TRF2) and histone H3K27 trimethylation (H3K27me3) immunohistochemistry in the diagnosis of endometrial atypical hyperplasia tissues. Methods A total of 30 specimens without endometrial atypical hyperplasia, 60 specimens with endometrial atypical hyperplasia, and 30 specimens with endometrioid carcinoma were collected in Shaoguan First People′s Hospital in Guangdong Province from March 2016 to November 2018. The immunohistochemical SP method was used to detect the expressions of TRF1, TRF2 and H3K27me3 in these specimens. Results The expression rates of TRF1, H3K27me3, and TRF2 in the group with atypical endometrial dysplasia were 35.00% (21/60), 43.33% (26/60), and 71.67% (43/60), respectively. The expression rates in the group without atypical endometrial hyperplasia were 83.33% (25/30), 73.33% (22/30), and 30.00% (9/30), respectively. The expression rates in the group with endometrioid carcinoma were 30.00% (9/30), 36.67% (11/30), and 76.67% (23/30), respectively. There were statistically significant differences in the expression rates between the group with atypical endometrial hyperplasia and the group without atypical endometrial hyperplasia (P<0.05). There were no statistically significant differences in the expression rates between the group with atypical endometrial hyperplasia and the group with endometrioid carcinoma (P>0.05). Conclusion TRF1 and H3K27me3 are low-expressed in endometrial atypical hyperplasia, and TRF2 is high-expressed. The combination of TRF1, TRF2 and H3K27me3 can help distinguish the group with endometrial atypical hyperplasia from the group without endometrial atypical hyperplasia, which is helpful in early diagnosis of endometrial atypical hyperplasia. It is recommended in routine pathological practice.

nlc202206161133



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